Coding

Part:BBa_K730002:Design

Designed by: Antresh Kumar   Group: iGEM12_JUIT-India   (2012-09-26)


The complete denitrification of nitrate by bacteria to dinitrogen (N,)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
    Illegal NgoMIV site found at 908
    Illegal NgoMIV site found at 1072
    Illegal AgeI site found at 282
    Illegal AgeI site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 667
    Illegal BsaI.rc site found at 1312


Design Notes

nosZ; nitrous-oxide reductase (EC:1.7.99.6); K00376 nitrous-oxide reductase [EC:1.7.2.4]


Source

Yes it comes from Pseudomonas aeruginosa

References